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p-1000 horizontal electrode puller  (Sutter Instrument Company)


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    Sutter Instrument Company p-1000 horizontal electrode puller
    P 1000 Horizontal Electrode Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p-1000 horizontal electrode puller/product/Sutter Instrument Company
    Average 90 stars, based on 1 article reviews
    p-1000 horizontal electrode puller - by Bioz Stars, 2026-06
    90/100 stars

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    High spatiotemporal resolution imaging of a single SypHy fusion events at single ribbon location in response to depolarization stimuli (A) Top : Synaptic terminals of retinal bipolar cells from transgenic zebrafish that sparsely express SypHy were voltage clamped by placing the patch pipette filled with Cy5-RBP. We generated rapid x-t line-scans at a ribbon location to obtain high spatiotemporal images of SypHy fusion events. We chose the ribbons that are sharp focused (For example 1, 2 or 5) and at the edge of the plasma membrane to generate line-scan. Bottom : x-t line-scans (white dash lines) were obtained across the ribbon, perpendicular to plasma membrane. (B) Left : Representation of a raw x-t raster plot of SypHy (green) and ribbon (red). The line-scans were obtained at 7.44 ms/line consisting of 2000-line scans. Right : TTL trigger from Patch Master computer delivered to the imaging computer to start image acquisition. In addition, <t>horizontal-scan</t> synch from imaging computer is delivered to Patch Master to obtain precise timing of image acquisition. Besides, voltage command, information about calcium current and capacitance measurements for each stimulus also can be obtained from Patch Master computer. (C) To enhance the SypHy fluorescence signal and optimize the SNR the same line scan in Figure 2B but averaged 2-pixels along x-axis and 5-pixels along time-axis.
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    Sutter Instrument Company horizontal p-1000 electrode puller
    High spatiotemporal resolution imaging of a single SypHy fusion events at single ribbon location in response to depolarization stimuli (A) Top : Synaptic terminals of retinal bipolar cells from transgenic zebrafish that sparsely express SypHy were voltage clamped by placing the patch pipette filled with Cy5-RBP. We generated rapid x-t line-scans at a ribbon location to obtain high spatiotemporal images of SypHy fusion events. We chose the ribbons that are sharp focused (For example 1, 2 or 5) and at the edge of the plasma membrane to generate line-scan. Bottom : x-t line-scans (white dash lines) were obtained across the ribbon, perpendicular to plasma membrane. (B) Left : Representation of a raw x-t raster plot of SypHy (green) and ribbon (red). The line-scans were obtained at 7.44 ms/line consisting of 2000-line scans. Right : TTL trigger from Patch Master computer delivered to the imaging computer to start image acquisition. In addition, <t>horizontal-scan</t> synch from imaging computer is delivered to Patch Master to obtain precise timing of image acquisition. Besides, voltage command, information about calcium current and capacitance measurements for each stimulus also can be obtained from Patch Master computer. (C) To enhance the SypHy fluorescence signal and optimize the SNR the same line scan in Figure 2B but averaged 2-pixels along x-axis and 5-pixels along time-axis.
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    High spatiotemporal resolution imaging of a single SypHy fusion events at single ribbon location in response to depolarization stimuli (A) Top : Synaptic terminals of retinal bipolar cells from transgenic zebrafish that sparsely express SypHy were voltage clamped by placing the patch pipette filled with Cy5-RBP. We generated rapid x-t line-scans at a ribbon location to obtain high spatiotemporal images of SypHy fusion events. We chose the ribbons that are sharp focused (For example 1, 2 or 5) and at the edge of the plasma membrane to generate line-scan. Bottom : x-t line-scans (white dash lines) were obtained across the ribbon, perpendicular to plasma membrane. (B) Left : Representation of a raw x-t raster plot of SypHy (green) and ribbon (red). The line-scans were obtained at 7.44 ms/line consisting of 2000-line scans. Right : TTL trigger from Patch Master computer delivered to the imaging computer to start image acquisition. In addition, horizontal-scan synch from imaging computer is delivered to Patch Master to obtain precise timing of image acquisition. Besides, voltage command, information about calcium current and capacitance measurements for each stimulus also can be obtained from Patch Master computer. (C) To enhance the SypHy fluorescence signal and optimize the SNR the same line scan in Figure 2B but averaged 2-pixels along x-axis and 5-pixels along time-axis.

    Journal: STAR Protocols

    Article Title: Tracking the dynamics of single fused synaptic vesicle proteins from a single ribbon active zone in zebrafish retinal bipolar cells

    doi: 10.1016/j.xpro.2021.101107

    Figure Lengend Snippet: High spatiotemporal resolution imaging of a single SypHy fusion events at single ribbon location in response to depolarization stimuli (A) Top : Synaptic terminals of retinal bipolar cells from transgenic zebrafish that sparsely express SypHy were voltage clamped by placing the patch pipette filled with Cy5-RBP. We generated rapid x-t line-scans at a ribbon location to obtain high spatiotemporal images of SypHy fusion events. We chose the ribbons that are sharp focused (For example 1, 2 or 5) and at the edge of the plasma membrane to generate line-scan. Bottom : x-t line-scans (white dash lines) were obtained across the ribbon, perpendicular to plasma membrane. (B) Left : Representation of a raw x-t raster plot of SypHy (green) and ribbon (red). The line-scans were obtained at 7.44 ms/line consisting of 2000-line scans. Right : TTL trigger from Patch Master computer delivered to the imaging computer to start image acquisition. In addition, horizontal-scan synch from imaging computer is delivered to Patch Master to obtain precise timing of image acquisition. Besides, voltage command, information about calcium current and capacitance measurements for each stimulus also can be obtained from Patch Master computer. (C) To enhance the SypHy fluorescence signal and optimize the SNR the same line scan in Figure 2B but averaged 2-pixels along x-axis and 5-pixels along time-axis.

    Article Snippet: Horizontal electrode puller , Sutter Instrument , P-1000.

    Techniques: Imaging, Transgenic Assay, Transferring, Generated, Clinical Proteomics, Membrane, Fluorescence

    Journal: STAR Protocols

    Article Title: Tracking the dynamics of single fused synaptic vesicle proteins from a single ribbon active zone in zebrafish retinal bipolar cells

    doi: 10.1016/j.xpro.2021.101107

    Figure Lengend Snippet:

    Article Snippet: Horizontal electrode puller , Sutter Instrument , P-1000.

    Techniques: Recombinant, Transgenic Assay, Control, Software, Inverted Microscopy, Patch Clamp